Viruses which can be cultured from blood include dengue viruses and some arboviruses. Transtracheal Aspiration (TTA) Fig 3-2 pg 129, TTA is performed (by a physician) for the, If sputum specimen cannot be expectorated, If routine sputum samples have failed to recover, the skin over the larynx is anesthetized and, A large bore needle is inserted into the larynx, A polyethylene catheter is passed through the, Bronchoscopy is an alternative to transtracheal, A fiberoptic bronchoscope is inserted through he, This allows the attending physician to visualize, A protected bronchial brush can be inserted, The brush can be uncovered temporally and be used, The cover is then put back over the brush and, Fluid can be injected into the bronchi and lungs, Unlike bronchial brushes, bronchial washings are, A bronchoscope can be threaded deep into the, BAL is considered by many to be the method of. Specimen Collection Suspect or confirmed TB patients should be in a negative pressure room Specimen collection is an aerosol generating procedure, anyone in the room during specimen collection must wear a particulate respirator type N-95 and be part of the respirator protection plan All mycobacteria specimens are collected Nasopharyngeal specimens are required to diagnose respiratory syncytial virus infection, and when necessary to investigate infections caused by influenza and parainfluenza viruses, measles, rubella virus, adenoviruses, and enteroviruses. The specimen should be collected in the morning to reach the laboratory before noon, so that it can be processed the same day. Name, place, age, number of the patient, and details of any recent travel. endstream endobj 364 0 obj <>/Metadata 31 0 R/Pages 361 0 R/StructTreeRoot 63 0 R/Type/Catalog>> endobj 365 0 obj <>/MediaBox[0 0 612 792]/Parent 361 0 R/Resources<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI]/XObject<>>>/Rotate 0/StructParents 0/Tabs/S/Type/Page>> endobj 366 0 obj <>stream <> The SlideShare family just got bigger. EQUIPMENTcon., Alcohol Wipes - 70% isopropyl alcohol. Enjoy access to millions of ebooks, audiobooks, magazines, and more from Scribd. The type of specimen, the appropriate time to obtain the sample, the way of sampling, the storage and transport are critical points in the diagnostic process. What specimens are to be collected? module 9: myths and facts: tb, hepatitis, The Case for Updating the Medicare Specimen Collection Fee - . - Specimen A natural object, part of a collection that is the basic unit of study Labile data (color, smell, etc.) Tube slips back from vacutainer needle. Check the requisition form for requested tests, patient information, and any special requirements. Methodologies and Regulations in Specimen Collection and Management - . Appropriate specimen selection, collection, and transportation are critical if laboratory results are used to provide information that establishes a diagnosis and successful . The SlideShare family just got bigger. endstream endobj 368 0 obj <>stream The availability of new laboratory techniques for unusual pathogens, makes necessary the review and update of all the steps involved in the processing of the samples. Name and address of the hospital or health centre. Appropriate shipping of specimen to the laboratory for testing. During vegetation sampling, collect repre- Normal reference ranges are often noted according to age. EDTA Side-Effects EDTA is hyperosmolar causing cell shrinkage but the low pH of EDTA counterbalances this effect because low pH causes cells to expand. <> We've updated our privacy policy. - Collection of Epidemiologic Data Bao-Ping Zhu, MD, MS - Title: Slide 1 Created Date: 11/20/2008 5:55:36 PM Document presentation format: On-screen Show (4:3) Other titles: Arial Majalla UI Calibri Traditional Arabic How Warring Medical Kits Provides Valued Protection for Specimen Collection. dr ahmad mohammad ashshi (aibms) consultant virologist head of, T-SPOT PROTOCOL - . Details about immunization and antimicrobial therapy. JFIF C | Learn more about . An exception to this rule, throat swabs for S. Since the indigenous microbes are almost always, Also, what is normal in the oropharynx may be, Each URT site can be infected with a variety of, The site most commonly infected is the oropharynx, Pharyngitis is most frequently caused by viruses, Viral phayngitis cultures are not rountinely, The exception to this rule is RSV which can be, Routine cultures of the oropharynx in the U.S.A, S. pyogenes pharyngitis is frequently associated, S. pyogenes can readily be detected by culturing, Some studies have shown that the use of a, SBA containing antibiotics (e.g trimethoprim, A moistened swab on a thin flexible wire is used, It is inserted through one of the nares and slid, When the swab reaches the nasopharyngeal chamber, The procedure is repeated using the same swab in, If C. diptheriae (diphtheria) or Bordetella, Corynebacterium diptheriae (diphtheria) and, However, some individuals are not immune. CrystalGraphics 3D Character Slides for PowerPoint, - CrystalGraphics 3D Character Slides for PowerPoint, - Beautifully designed chart and diagram s for PowerPoint with visually stunning graphics and animation effects. The most important aspects of microbiological testing are collection of the right specimen and transport of the specimen to lab in a manner that insure the reliability of the diagnostic procedure (e.g., culture, microscopy and antigen or antibody tests). Looks like youve clipped this slide to already. Specimen collection details: (Adapted from the WHO guidelines on 2019-nCoV): materials swabs* aspirate or nasal Serum *For transport of samples for viral detection, use VTM (viral transport medium) containing antifungal and antibiotic supplements. What is the collection order of tubes? Sample should be collected aseptically. Midstream "Clean-Catch" Urine Specimen 4.3. Type of specimen, if two, mention as the first of second. Specimen collection and transport Dr.Ashna Ajimsha Ashna Ajimsha 371 views Sample Collection In Microbiology Anuj Sharma 44.3k views Sample collection and safety procedure in laboratory Priyanka Buragohain 16k views Specimen collection and preservation Serene Pearl 48.1k views Similar to Collection and transport (20) SBA is included as well to increase recovery. Specimen labelling and processing: E E ROCE SSING specimens, SPECIMEN HANDLING & PATHOLOGY REPORT BEFORE AND AFTER BREAST CANCER NEOADJUVANT TREATMENT - . Smears of expectorated sputum should be made. Impact of Incorrect Volume on PT and APTT The APTT is the most sensitive to variations in final citrate concentration. 393 0 obj <>stream In general, specimens for virus isolation should be collected within 4 days after onset of illness as virus shedding decreases rapidly after that time. He/She verifies all orders, procedures, and specimens according to current procedures. Critically timed specimens (e.g. Frozen specimens thawing during transport b. Unspun specimens transported >2 hours from collection Specimen Collection Patient results are only as good as the specimen collected. Boasting an impressive range of designs, they will support your presentations with inspiring background photos or videos that support your themes, set the right mood, enhance your credibility and inspire your audiences. Patients Preparation factors Therapeutic Drug Monitoring: different pharmacologic agents have patterns of administration, body distribution, metabolism, and elimination that affect the drug concentration as measured in the blood. (Partial penetration may allow blood to leak into the soft tissue surrounding the vein by way of the needle bevel) Remove the tourniquet before removing the needle Use the major superficial veins Apply pressure to the venipuncture site Avoid a probing, traumatic venipuncture, To prevent hemolysis (which can interfere with many tests): Mix tubes with anticoagulant additives gently 5-10 times. Check for timing instructions giving by the physician for drawing the appropriate samples. Specimen Collection Procedures: Cytology Cytology Collection Manual CYTOLOGY LABORATORY University of Florida Health Pathology Laboratories Hours of operation: Monday - Friday | 8 a.m. - 4:30 p.m. Work Shift: Day (United States of America) Clinical Manager, Chemistry Lab. Faecal specimens should be collected in the early stages of the diarrhoeal disease, when pathogens are present in the highest number, and preferably before antimicrobial treatment is started, if appropriate. Blood for antibody testing should be collected as early in the disease as possible and also 5- 10 days later. Proper specimen labeling. - Prefilled Specimen Containers is a convenient method for both physicians and medical professionals to collect transport and preserve blood, platelets and plasma for long-term study or research. 4 0 obj NE P S OR SP CIM N Specimen Collection and Processing - Free download as Powerpoint Presentation (.ppt / .pptx), PDF File (.pdf), Text File (.txt) or view presentation slides online. Instant access to millions of ebooks, audiobooks, magazines, podcasts and more. It's FREE! Thats why, the lab staff should be informed about the kind of medication ( e.g. objective. Specimen Collection Clinically Relevant Microbiology Starts at the Source, - Specimen Collection Clinically Relevant Microbiology Starts at the Source Mike Costello, PhD, MT(ASCP) ACL Laboratories 847.349.7403 mike.costello@advocatehealth.com. Gloves - can be made of latex, rubber, vinyl, etc. ",#(7),01444'9=82. Instant access to millions of ebooks, audiobooks, magazines, podcasts and more. ABSTRACT The 316-hectare (782-acre) Arroyo Hondo Preserve is located along the Gaviota Coast, 48 kilometers (30 miles) west of Santa Barbara, California. Tourniquet - Wipe off with alcohol and replace frequently. Many blood-based analyses require collection into yellow-topped acid-citrate-dextrose tubes and overnight shipment of the chilled tubes to the laboratory. Template SPECIMEN Adhesive bandages / tape - protects the venipuncture site after collection, Collection Tube Additives Heparin EDTA Citrate Clot Activator Serum Separator, Blood should NEVER be poured from one tube to another since the tubes can have different additives or coatings. endstream Specimen Collection and Laboratory Diagnosis of Lower, - Specimen Collection and Laboratory Diagnosis of Lower Respiratory Infections Mohammad Rahbar (PhD) Department Of Microbiology Reference Laboratory of Iran, Principles of specimen collection in pathology. - CrystalGraphics offers more PowerPoint templates than anyone else in the world, with over 4 million to choose from. malak m. el-hazmi. The most important aspects of microbiological testing are collection of the right specimen and transport of the specimen to lab in a manner that insure the reliability of the diagnostic procedure (e.g., culture, microscopy and antigen or antibody tests). f47ztf9!oKBt-~Um93V3)7u;ZF-#H1M For body fluid collection provide the patient with the suitable container and collection instruction . %PDF-1.5 Special specimen collection procedures, including more invasive procedures, or those with an increased risk of complications to the patient, (e.g. z.$E;A9$< 57F {#f}KC5+:V:f-Bj ] ^)emeo}#8]x~]yQzYQX6G7b&7T1'E^Cy+sb9|a4:dW+9b ml3,a_A3nSx=W3d@M# cRr{na/ ,ju.Zje^-F38x% &c&^o3XhG(b3xz?I%~c5/-\^q%,n._| Y+ - PowerPoint PPT presentation Number of Views: 6325 Avg rating:3.0/5.0 Slides: 45 Provided by: UCCB Category: Tags: collection | specimen less Weve updated our privacy policy so that we are compliant with changing global privacy regulations and to provide you with insight into the limited ways in which we use your data. Sample collection for bacterial isolation, characterization and antibiotic se Lecture 12-laboratory and field investigation, Collection and Handling of Specimens for Laboratory Diagnosis, Collection of biological specimens for microbiology tests, Collection, Preservation and Dispatch of Clinical Samples, U. P. Veterinary University (DUVASU), Mathura, Culturing of microbiology specimens in laboratories, Medical Microbiology Laboratory (sample collection), Collection, transport & storage of clinical specimens, Collection and transport of clinical samples, specimen collection&handling,Lusubilo2012, Microbiological examination of urine sample, Processing of urine in microbiology by Rahul raj, Specimen collection and transport Dr.Ashna Ajimsha, Sample collection and safety procedure in laboratory, Laboratory diagnosis of_infectious_diseases, Collection and transport of clinical samples in laboratorries, Specimen collection 130319145806-phpapp02, Lab sample collection techniques pathology, COLLECTION AND TRANSPORTATION OF CLINICAL SAMPLES, Specimen collection for clinical microbiology laboratory, 2021 laboratory diagnosis of infectious diseases dr.ihsan alsaimary, 21 and 22 SME FINANCE Stressed Asset Management.pptx, 21 and 22 SME FINANCE Stressed Asset Management and Recovery.pptx, Pitch Deck Teardown: Scrintal's $1M Seed deck, No public clipboards found for this slide, Enjoy access to millions of presentations, documents, ebooks, audiobooks, magazines, and more. 379 0 obj <>/Filter/FlateDecode/ID[<0418542909B6434FA5DC5F39696DAE8B>]/Index[363 31]/Info 362 0 R/Length 89/Prev 695974/Root 364 0 R/Size 394/Type/XRef/W[1 3 1]>>stream <>/XObject<>/Font<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 720 540] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> microbiology. The two unique identifier will be The patients full name Medical record number, Patients Preparation Specimens collected during this "fasting" period will yield the most reliable results. Two to. It increases by an average of 3% for 10% underfilling and 10% for a 20% underfilling. Laboratory specimen: collection, safe transport and biosafety. Completing each step correctly improves the likelihood the specimen can be successfully tested. Heparin (Anti-Coagulant) Used to collect whole blood or plasma Binds to anti-thrombin III to inhibit Xa, IXa, and thrombin Nominal concentration of 12 30 U/mL Heparin binds calcium so ionized calcium must be collected using Calcium Titrated or Electrolyte Balanced heparin, Citrate (Anti-Coagulant) Citrate is used for collection of coagulation tests Acts by binding calcium Nominal concentration of 3.2% (0.109 mol/L) Recent move to 3.2% from 3.8% to get more consistent results for Prothrombin Time, particularly for more sensitive reagents Tubes must be properly filled to within +/- 10% of assigned collection volume, Clot Activator Shortens Time to Clot In vitro activation of clotting system to enhance clot formation A silica clot activator is attached to the tube wall with a silicone surfactant Requires mixing for optimal affect Time to clot is 15-30 minutes instead of 1 hour, Serum Separator Gel Separates Serum from Cells Polymer gel with specific gravity between that of serum and cells Migrates and forms a barrier during centrifugation Separation of cells from serum stops metabolic and hemolytic effects Certain analytes and therapeutic drugs may bind to gel over time, Labelling the Sample A properly labelled sample is essential so that the results of the test match the patient. 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